Add Proteomic Evaluation Of Lysine Acetylation Sites In Rat Tissues Reveals Organ Specificity And Subcellular Patterns

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NOTE: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and also a few of the amino teams within the spot areas don't type bonds with the amino acids. BE AWARE: This avoids the coupling of amino acids within the additional cycles with the membrane instead of the growing peptide chain. Ensure all the purification buffers are at 4 °C and perform the protein purification in the cold room to keep away from protein denaturation.
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